Search results for " cell-line"
Article
Best Practices for Selecting a Top-Quality Cell Line
Given the number of potential factors that can influence cell-line quality, the increasing complexity of biologic drug substances and growing regulatory requirements, identifying the optimum cell line…
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Generating a Fully Processed Antibody
Producing these multisubunit molecules or processing proteins in the correct proportions to obtain maximum titer and biopharmaceutical quality can be difficult using traditional cell-line development …
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A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Early-phase assessment includes characterization of the expression plasmid prior to cell-line generation (transfection); evaluation of transcript integrity of those transgenes expressed transiently an…
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Cell-Culture Advances Test Bioreactor Performance Models
…n transfer rate, oxygen uptake rate, carbon dioxide evolution, carbon dioxide stripping, metabolism, cell-line traits, etc.
In addition, Smith notes that improvements in understanding of cell b…
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Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
While it is possible to weed the poorly performing cell lines out during the cell-line selection process, it is desirable to have a platform process that is as broadly applicable as possible. To this …
Article
Platform Approach Speeds Process Development
The GS-system, in contrast, enables the cultivation on cells without additional selective pressure in media and enables reduced cell-line development (CLD) timelines. All steps during CLD, including t…
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Changing the Culture of Cell Culture: Applying Best Practices and Authentication to Ensure Scientific Reproducibility
Another DNA profiling test of cell-line identity uses single nucleotide polymorphism (SNP) variations between members of the same species within a specific locus, which are conserved during evolution …
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Maximum Output Starts with Optimized Upstream Processing
Selexis and KBI Biopharma, for instance, have invested in technology and innovation in cell-line development and process development to reduce cost of goods while increasing output at a given scale. H…
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Commercial Production of Gene Therapies Using Suspension or Adherent Cell Lines
This article will explore the traditional path from the laboratory to the clinic and how the fixed-bed technology provides an alternative solution to meet commercial demands.
By Alex Chatel and …
Article
Gene Therapies Push Viral Vector Production
That being said, other technologies such mRNAs delivered to the target cells with, for example, mammalian cell-line produced engineered exosomes, start to show their capabilities,” Touw interjects. “A…